CD33 antibody

Catalog No. ABIN120672

This anti-CD33 antibody is a Mouse Monoclonal antibody detecting CD33 in FACS. Suitable for Human.

Quick Overview for CD33 antibody (ABIN120672)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HIM3-4

Product Details anti-CD33 Antibody

Specificity: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Purification: Protein-G affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: NFMY-9s human cell line

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD33

Target: CD33

Alternative Name: CD33 / SIGLEC3

Background: CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID: 945

UniProt: P20138